Modulating oxidative stress, apoptosis, and mitochondrial dysfunctions on cardiotoxicity induced by aluminum phosphide pesticide using resveratrol.

The agricultural fumigant pesticide aluminum phosphide (AlP) is cardiotoxic. Water causes AlP to emit phosphine gas, a cardiac toxin that affects heart function and causes cardiogenic shock. AlP poisoning's high fatality rate is due to cardiotoxicity. This study examines how resveratrol reduces oxidative stress, mitochondrial activity, and apoptosis in human cardiac myocyte (HCM) cells. After determining the optimal doses of resveratrol using the MTT test, HCM cells were subjected to a 24-h treatment of resveratrol following exposure to AlP (2.36 µM). The levels of reactive oxygen species (ROS), superoxide dismutase (SOD) activity, mitochondrial swelling, mitochondrial cytochrome c release, and mitochondrial membrane potential (MMP) in HCM cells were investigated. Also, the expression of Bax and Bcl-2, caspace-3 activity, and apoptosis were assessed. The present investigation revealed that AlP substantially increased the level of ROS and decreased SOD activation, which were significantly modulated by resveratrol in a dose-dependent manner. Moreover, AlP induced an elevation of mitochondrial swelling, cytochrome c release, and MMP collapse. Co-administration of resveratrol significantly reduced above mitochondrial markers. AlP also significantly upregulated BAX and downregulated Bcl-2 expression, elevated caspace-3 activity, and apoptosis. Resveratrol co-administration was able to meaningfully modulate the mentioned parameters and finally reduce apoptosis. In conclusion, resveratrol, via its pleotropic properties, significantly demonstrated cytoprotective effects on HCM cytotoxicity induced by AlP.

Versatility of copper-iron bimetallic nanoparticles fabricated using Hibiscus rosa-sinensis flower phytochemicals: various enzymes inhibition, antibiofilm effect, chromium reduction and dyes removal.

Bimetallic nanoparticles (NPs) are considered superior in terms of stability and function with respect to its monometallic counterparts. Hence, in the present study Hibiscus rosa-sinensis flower extract was used to synthesis copper-iron bimetallic nanoparticles (HF-FCNPs). HF-FCNPs was characterized and its applications (biological and environmental) were determined. HF-FCNPs were spherical in shape with high percentage of copper inducted into the NPs. HF-FCNPs inhibited mammalian glucosidases [maltase (IC50: 548.71 ± 61.01 µg/mL), sucrase (IC50: 441.34 ± 36.03 µg/mL), isomaltase (IC50: 466.37 ± 27.09 µg/mL) and glucoamylase (IC50: 403.12 ± 14.03 µg/mL)], alpha-amylase (IC50: 16.27 ± 1.73 µg/mL) and acetylcholinesterase [AChE (IC50: 0.032 ± 0.004 µg/mL)] activities. HF-FCNPs showed competitive inhibition against AChE, maltase and sucrase activities; mixed inhibition against isomaltase and glucoamylase activities; whereas non-competitive inhibition against α-amylase activity. HF-FCNPs showed zone of inhibition of 16 ± 2 mm against S. mutans at 100 µg/mL concentration. HF-FCNPs inhibited biofilm formation of dental pathogen, S. mutans. SEM and confocal microscopy analysis revealed the disruption of network formation and bacterial cell death induced by HF-FCNPs treatment on tooth model of S. mutans biofilm. HF-FCNPs efficiently removed hexavalent chromium in pH-independent manner and followed first order kinetics. Through Langmuir isotherm fit the qmax (maximum adsorption capacity) was determined to be 62.5 mg/g. Further, HF-FCNPs removed both anionic and cationic dyes. Altogether, facile synthesis of HF-FCNPs was accomplished and its biological (enzyme inhibition and antibiofilm activity) and environmental (catalyst to remove pollutants) applications have been understood.